IDE-1 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells1. IDE-1 is able to generate Sox17+/FoxA2+ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) inducers of definitive endoderm in mouse or human ES cell cultures1. It has an EC50 of 125.5 nM and functions in part via activation of TGF-beta² signaling, as evident by Smad2 phosphorylation1. Endoderm chemically induced by IDE-1 can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos1
Product Specification
M.Wt: 306.31
Formula:C15H18N2O5
Solubility:Soluble to 100 mM in DMSO
Purity:>98 %Storage:
CAS No:[1160927-48-9]
Borowiak, M., Maehr, R., Chen, S., Chen, A.E., Tang, W., Fox, J.L., Schreiber, S.L., Melton, D.A. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4: 348-58
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